GCSE Biology Practice Test 7 – 60 Questions with Answers (AQA 8461)
Work through all 60 GCSE questions, checking each answer and explanation as you go.
Cell biology
Q1. What is the function of ribosomes in a cell?
- (A) To make proteins
- (B) To release energy
- (C) To store genetic information
- (D) To control substances entering the cell
Show Answer
Answer: A – Ribosomes are the site of protein synthesis.
Q2. Which structure contains the genetic material that controls cell activities?
- (A) Chloroplast
- (B) Nucleus
- (C) Cell wall
- (D) Permanent vacuole
Show Answer
Answer: B – The nucleus contains DNA, which controls the activities of the cell.
Q3. What is the function of the large permanent vacuole in a plant cell?
- (A) To carry out photosynthesis
- (B) To release energy by respiration
- (C) To store cell sap and help support the cell
- (D) To control cell division
Show Answer
Answer: C – The vacuole contains cell sap and, when full, keeps the cell turgid and supported.
Q4. A microscope image of a cell is 12 mm long. The real cell is 0.02 mm long. What is the magnification?
- (A) 600
- (B) 60
- (C) 240
- (D) 6000
Show Answer
Answer: A – Magnification equals image size divided by real size: 12 divided by 0.02 equals 600.
Q5. Stem cells in plant meristems are able to do what?
- (A) Produce only root hair cells
- (B) Carry out photosynthesis in leaves only
- (C) Differentiate into any type of plant cell
- (D) Divide only once in the life of the plant
Show Answer
Answer: C – Meristem stem cells can differentiate into all plant cell types.
Q6. Why is mitosis important for a growing organism?
- (A) It produces gametes with half the chromosome number
- (B) It produces new identical cells for growth and replacement
- (C) It creates genetic variation in offspring
- (D) It reduces the number of chromosomes in body cells
Show Answer
Answer: B – Mitosis adds identical cells for growth, repair and replacement.
Q7. How does increasing temperature usually affect the rate of diffusion?
- (A) It decreases the rate because particles slow down
- (B) It has no effect on particle movement
- (C) It increases the rate because particles move faster
- (D) It stops diffusion completely
Show Answer
Answer: C – Higher temperature gives particles more kinetic energy, so they move and spread faster.
Q8. A plant cell is placed in pure water. What happens to it?
- (A) Water enters by osmosis, the cell becomes turgid, and the cell wall prevents it bursting
- (B) Water leaves by osmosis and the cell shrinks
- (C) The cell bursts because it has no cell wall
- (D) Nothing happens because plant cells cannot absorb water
Show Answer
Answer: A – Water enters the vacuole by osmosis, but the strong cell wall stops the cell bursting.
Q9. Mineral ions in soil water are at a lower concentration than inside a root hair cell. How are they taken up?
- (A) By active transport, using energy
- (B) By diffusion down a gradient
- (C) By osmosis across the membrane
- (D) By photosynthesis in the root
Show Answer
Answer: A – Because the ions move against their concentration gradient, energy is needed for active transport.
Organisation
Q10. Why is the stomach acidic?
- (A) The acid breaks down starch into sugars
- (B) The acid neutralises bile
- (C) The acid absorbs amino acids into the blood
- (D) The acid kills many pathogens and provides the optimum pH for protease enzymes
Show Answer
Answer: D – Hydrochloric acid in the stomach kills pathogens and gives pepsin its optimum pH.
Q11. Which adaptation of villi in the small intestine increases absorption?
- (A) A large surface area, a thin wall and a rich blood supply
- (B) A thick wall and a small surface area
- (C) The presence of a large nucleus
- (D) The ability to produce bile
Show Answer
Answer: A – Villi increase surface area, have thin walls for short diffusion distances and a good blood supply.
Q12. Bile is stored in the gall bladder. What does bile do in digestion?
- (A) It breaks down protein
- (B) It produces insulin
- (C) It helps absorb vitamins
- (D) It emulsifies fats and neutralises stomach acid
Show Answer
Answer: D – Bile breaks large fat droplets into smaller ones for lipase and provides an alkaline pH.
Q13. What is meant by a double circulatory system?
- (A) Blood passes through the heart twice for each complete circuit of the body
- (B) The heart has two chambers only
- (C) Blood flows in both directions in every vessel
- (D) There are two separate hearts in the body
Show Answer
Answer: A – In a double circulation, blood goes through the heart once on its way to the lungs and once on its way to the body.
Q14. Capillaries are very narrow, so red blood cells pass through in single file. What advantage does this give?
- (A) Blood flows faster through narrow vessels
- (B) Each red blood cell is close to tissue cells for rapid diffusion
- (C) Pressure stays high in the tissues
- (D) Blood can clot more easily
Show Answer
Answer: B – Single-file flow keeps every red blood cell close to the capillary wall, shortening the diffusion distance.
Q15. Why does blood pressure fall as blood moves from arteries through capillaries?
- (A) Valves block the flow of blood
- (B) The heart stops beating between pulses
- (C) Blood is filtered by the kidneys
- (D) The total cross-sectional area of the vessels increases
Show Answer
Answer: D – Blood spreads through many narrow vessels, so pressure drops even though total flow continues.
Q16. Which vessel carries blood from the heart to the rest of the body?
- (A) The pulmonary artery
- (B) The vena cava
- (C) The pulmonary vein
- (D) The aorta
Show Answer
Answer: D – The aorta carries oxygenated blood from the left ventricle to the body tissues.
Q17. Which plant tissue transports dissolved sugars, and in which directions?
- (A) Xylem, both up and down the plant
- (B) Phloem, both up and down the plant
- (C) Phloem, upwards only
- (D) Xylem, downwards only
Show Answer
Answer: B – Phloem carries sugars made in leaves to all parts of the plant, up and down.
Q18. How is a root hair cell adapted for absorbing water?
- (A) It contains many chloroplasts
- (B) It has a thick waterproof wall
- (C) It contains a large permanent vacuole only
- (D) Its long extension gives a large surface area
Show Answer
Answer: D – The root hair extension greatly increases the surface area for water uptake.
Infection and response
Q19. How do many bacteria damage the body?
- (A) They enter cells and use them to make copies of themselves
- (B) They block nerve impulses in the brain
- (C) They reproduce rapidly and may produce toxins
- (D) They destroy red blood cells by phagocytosis
Show Answer
Answer: C – Bacteria divide quickly and some release toxins that damage tissues.
Q20. How does a virus reproduce?
- (A) By dividing in two outside a host cell
- (B) By producing spores in the air
- (C) By feeding on dead material in the soil
- (D) By taking over a living host cell and using it to make new viruses
Show Answer
Answer: D – Viruses must infect a living cell, where they hijack its machinery to replicate.
Q21. Athletes foot is caused by which type of pathogen?
- (A) A bacterium
- (B) A virus
- (C) A fungus
- (D) A protist
Show Answer
Answer: C – Athletes foot is a fungal infection spread by contact with infected skin or surfaces.
Q22. Tobacco mosaic virus affects plants by doing what?
- (A) It causes roots to rot in wet soil
- (B) It makes leaves produce extra chlorophyll
- (C) It increases the rate of transpiration only
- (D) It discolours leaves and reduces photosynthesis, stunting growth
Show Answer
Answer: D – Tobacco mosaic virus causes mottled, discoloured leaves, so less photosynthesis occurs.
Q23. What do antitoxins do?
- (A) They neutralise toxins produced by bacteria
- (B) They engulf pathogens whole
- (C) They produce antibodies against viruses
- (D) They kill fungi on the skin
Show Answer
Answer: A – Antitoxins counteract bacterial toxins in the blood.
Q24. After infection, some lymphocytes remain as memory cells. What is their role?
- (A) They engulf pathogens
- (B) They make the skin thicker
- (C) They recognise the same pathogen quickly on reinfection and trigger fast antibody production
- (D) They produce antibiotics
Show Answer
Answer: C – Memory cells allow a rapid secondary immune response if the same pathogen returns.
Q25. How is measles mainly spread between people?
- (A) By drinking contaminated water
- (B) By mosquito bites
- (C) By droplets breathed out by an infected person and inhaled by others
- (D) By contact with soil
Show Answer
Answer: C – Measles virus spreads in droplets from coughs and sneezes.
Q26. Penicillin was discovered from which source?
- (A) A bacterium found in soil
- (B) A virus that infects fungi
- (C) A mould of the genus Penicillium
- (D) A plant root extract
Show Answer
Answer: C – Alexander Fleming noticed that Penicillium mould produced a substance that killed bacteria.
Q27. In a clinical trial, why is a placebo used?
- (A) To compare the effect of the drug with no active treatment and check for real improvement
- (B) To make the drug taste better
- (C) To double the dose given to patients
- (D) To replace the need for safety testing
Show Answer
Answer: A – A placebo group shows whether improvement is due to the drug rather than expectation or natural recovery.
Bioenergetics
Q28. If carbon dioxide concentration is increased, what usually happens to the rate of photosynthesis at first?
- (A) It decreases immediately
- (B) It increases, until another factor becomes limiting
- (C) It stays the same at all concentrations
- (D) It stops because carbon dioxide is toxic to leaves
Show Answer
Answer: B – More carbon dioxide allows faster photosynthesis until light or temperature limits the rate.
Q29. What is the role of chlorophyll in photosynthesis?
- (A) It stores glucose as starch
- (B) It absorbs light energy for photosynthesis
- (C) It opens the stomata
- (D) It transports water in the xylem
Show Answer
Answer: B – Chlorophyll in chloroplasts absorbs light energy, which drives photosynthesis.
Q30. Glucose made in photosynthesis can be used to make which storage carbohydrate in plants?
- (A) Cellulose
- (B) Starch
- (C) Protein
- (D) Fat
Show Answer
Answer: B – Plants convert glucose to starch for storage in roots, seeds and leaves.
Q31. Which statement about aerobic and anaerobic respiration is correct?
- (A) Anaerobic respiration releases more energy than aerobic respiration
- (B) Both release exactly the same amount of energy
- (C) Aerobic respiration releases much more energy per glucose molecule than anaerobic respiration
- (D) Aerobic respiration produces lactic acid
Show Answer
Answer: C – Aerobic respiration transfers far more energy from each glucose molecule.
Q32. During anaerobic respiration in muscles, what is produced in addition to a small amount of energy?
- (A) Ethanol and carbon dioxide
- (B) Lactic acid
- (C) Glucose and oxygen
- (D) Water only
Show Answer
Answer: B – Anaerobic respiration in muscles breaks glucose down to lactic acid, releasing less energy than aerobic respiration.
Q33. Which of these is part of metabolism?
- (A) Digesting starch in the mouth only
- (B) Converting glucose to glycogen in the liver
- (C) Absorbing water in the large intestine
- (D) Pumping blood around the body
Show Answer
Answer: B – Metabolism is the sum of chemical reactions, including building glycogen from glucose.
Q34. A lamp is moved so it is twice as far from a pondweed plant. What happens to light intensity at the plant?
- (A) It falls to one quarter of its previous value
- (B) It doubles
- (C) It halves
- (D) It stays the same
Show Answer
Answer: A – Light intensity follows an inverse square relationship with distance, so doubling distance quarters intensity.
Q35. Why does bread dough rise when yeast is added?
- (A) Yeast produces oxygen for the dough
- (B) Yeast breaks down starch into lactic acid
- (C) Yeast respires anaerobically and releases carbon dioxide gas
- (D) Yeast absorbs water and swells up
Show Answer
Answer: C – Carbon dioxide from yeast fermentation forms bubbles that make dough rise.
Homeostasis and response
Q36. How do nervous and hormonal responses differ?
- (A) Nervous responses travel in the blood; hormonal responses travel along neurones
- (B) Hormonal responses are always faster than nervous responses
- (C) Nervous responses affect the whole body; hormonal responses affect one cell only
- (D) Nervous responses are fast and short-lived; hormonal responses are slower and longer-lasting
Show Answer
Answer: D – Nerve impulses are rapid and brief, while hormones travel in blood and act over a longer time.
Q37. Which receptor detects light?
- (A) Receptors in the retina of the eye
- (B) Receptors in the skin
- (C) Receptors in the ear
- (D) Receptors in the tongue
Show Answer
Answer: A – Light receptors in the retina respond to light intensity and colour.
Q38. What is an effector in a nervous response?
- (A) A receptor that detects a stimulus
- (B) A neurone in the spinal cord
- (C) A hormone in the blood
- (D) A muscle or gland that carries out the response
Show Answer
Answer: D – Effectors, muscles and glands, produce the response to a stimulus.
Q39. In the menstrual cycle, what triggers ovulation?
- (A) A fall in progesterone
- (B) A rise in testosterone
- (C) A surge in insulin
- (D) A surge in luteinising hormone (LH)
Show Answer
Answer: D – The LH surge around day 14 causes an egg to be released from the ovary.
Q40. What happens if progesterone levels fall at the end of the menstrual cycle?
- (A) The uterus lining breaks down and is lost during menstruation
- (B) Ovulation occurs immediately
- (C) The uterus lining thickens further
- (D) An egg is released from the ovary
Show Answer
Answer: A – Progesterone maintains the uterus lining; when it falls, the lining is shed.
Q41. What is a main function of the kidney?
- (A) To produce insulin
- (B) To digest proteins
- (C) To store urine until it is released
- (D) To filter blood and remove urea, excess ions and excess water
Show Answer
Answer: D – The kidney filters blood, reabsorbs useful substances and excretes urea and excess substances as urine.
Q42. How does shivering help maintain body temperature in the cold?
- (A) It increases heat loss from the skin
- (B) It stops blood flowing to muscles
- (C) Muscle contractions generate heat
- (D) It causes sweating
Show Answer
Answer: C – Involuntary muscle contractions in shivering release heat energy.
Q43. Which plant hormone causes fruit to ripen?
- (A) Auxin
- (B) Gibberellin
- (C) Ethene
- (D) Insulin
Show Answer
Answer: C – Ethene is a plant hormone that triggers ripening of fruit.
Inheritance, variation and evolution
Q44. In DNA, which bases pair together?
- (A) Adenine with guanine, and cytosine with thymine
- (B) Adenine with cytosine, and guanine with thymine
- (C) All bases pair with any other base
- (D) Adenine with thymine, and cytosine with guanine
Show Answer
Answer: D – Base pairing is specific: A pairs with T and C pairs with G.
Q45. How many chromosomes are there in a normal human body cell and in a gamete?
- (A) 23 in a body cell and 46 in a gamete
- (B) 46 in a body cell and 23 in a gamete
- (C) 46 in both body cells and gametes
- (D) 23 in both body cells and gametes
Show Answer
Answer: B – Body cells are diploid with 46 chromosomes; gametes are haploid with 23.
Q46. A homozygous dominant parent (AA) is crossed with a homozygous recessive parent (aa). What are all the offspring genotypes?
- (A) All AA
- (B) All aa
- (C) All Aa
- (D) Half AA and half aa
Show Answer
Answer: C – Each offspring receives A from one parent and a from the other, so all are Aa.
Q47. For a child to have cystic fibrosis, what must be true about the parents?
- (A) Only the mother needs to carry the allele
- (B) Each parent must pass on a recessive allele for cystic fibrosis
- (C) Only the father needs to carry the allele
- (D) Neither parent needs to carry the allele
Show Answer
Answer: B – Cystic fibrosis is recessive, so an affected child inherits the allele from both parents, who are usually carriers.
Q48. A plant with genotype Tt has tall stems, where T is tall and t is dwarf. What does this show about the T allele?
- (A) T is dominant because its effect shows with only one copy
- (B) T is recessive because it is hidden by t
- (C) T is codominant with t
- (D) T has been changed by mutation
Show Answer
Answer: A – A dominant allele is expressed in the phenotype even when only one copy is present.
Q49. Most mutations have no noticeable effect on an organism. Which outcome is also possible?
- (A) Every mutation kills the organism
- (B) Mutations always improve survival
- (C) Mutations are always passed to offspring
- (D) A mutation can occasionally give an advantage that natural selection favours
Show Answer
Answer: D – Most mutations are neutral, some are harmful, and a few give an advantage in a particular environment.
Q50. Which of these is caused by the environment only, not by genes?
- (A) Eye colour
- (B) A scar from an injury
- (C) Blood group
- (D) Natural hair colour
Show Answer
Answer: B – A scar results from an environmental event and is not inherited.
Q51. What evidence from fossils supports the theory of evolution?
- (A) Fossils prove that all species appeared at the same time
- (B) Fossils show that organisms never change
- (C) Fossils contain DNA from living species only
- (D) Fossils show species that no longer exist and gradual changes in organisms over time
Show Answer
Answer: D – The fossil record shows extinct species and changes over long periods, supporting evolution.
Ecology
Q52. In the food chain grass -> rabbit -> fox, what is the rabbit?
- (A) A producer
- (B) A secondary consumer
- (C) A primary consumer
- (D) A decomposer
Show Answer
Answer: C – The rabbit eats the producer, so it is a primary consumer and herbivore.
Q53. In a food chain, grass is eaten by rabbits and rabbits are eaten by foxes. If fox numbers fall sharply, what is the likely short-term effect on rabbits?
- (A) Rabbit numbers fall because foxes protect them
- (B) Rabbit numbers stay exactly the same
- (C) Rabbit numbers rise because fewer are eaten, and then grass decreases
- (D) Grass increases forever
Show Answer
Answer: C – With less predation, rabbit numbers rise, so more grass is eaten and grass cover falls.
Q54. Which processes return water vapour to the atmosphere in the water cycle?
- (A) Evaporation and transpiration
- (B) Condensation and precipitation
- (C) Filtration and absorption
- (D) Photosynthesis and respiration only
Show Answer
Answer: A – Water evaporates from surfaces and is lost from leaves by transpiration.
Q55. How does decay contribute to the carbon cycle?
- (A) Decomposers respire and release carbon dioxide
- (B) Decomposers absorb carbon dioxide for photosynthesis
- (C) Decay stores carbon permanently in soil
- (D) Decay converts carbon dioxide into oxygen
Show Answer
Answer: A – Microorganisms decomposing dead material respire, returning carbon dioxide to the air.
Q56. Why is biodiversity important in an ecosystem?
- (A) It guarantees no species will ever become extinct
- (B) It increases the number of predators only
- (C) It prevents all disease
- (D) Greater variety makes ecosystems more stable and resilient to change
Show Answer
Answer: D – Diverse ecosystems are better able to withstand environmental change.
Q57. Peat bogs are important for the carbon cycle because they do what?
- (A) They release oxygen for photosynthesis
- (B) They store large amounts of carbon in partly decayed plant material
- (C) They absorb methane from cattle
- (D) They prevent rainfall reaching rivers
Show Answer
Answer: B – Peat locks up carbon; draining or burning bogs releases it as carbon dioxide.
Q58. Acid rain is caused mainly by which pollutant from burning fossil fuels?
- (A) Carbon monoxide
- (B) Sulfur dioxide
- (C) Methane
- (D) Oxygen
Show Answer
Answer: B – Sulfur dioxide dissolves in rain to form acidic rain that damages trees and lakes.
Q59. What is the purpose of a captive breeding programme?
- (A) To breed animals for food production
- (B) To increase numbers of an endangered species so individuals can later be released
- (C) To keep animals in zoos permanently
- (D) To introduce new predators to an area
Show Answer
Answer: B – Captive breeding builds up endangered populations for reintroduction to the wild.
Q60. When would a scientist use a transect rather than a single quadrat?
- (A) To study how distribution changes along a gradient, such as down a seashore
- (B) To count every organism in a habitat
- (C) To measure the height of trees
- (D) To sample animals that move quickly
Show Answer
Answer: A – A transect line with quadrats along it shows change across an environmental gradient.
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