Chromatographic Techniques – HPLC & Ion Exchange Chromatography MCQs
Chromatographic techniques like HPLC (High-Performance Liquid Chromatography) and Ion Exchange Chromatography are essential for separating, identifying, and purifying compounds in chemistry, pharmaceuticals, and biochemistry. HPLC uses high pressure to enhance separation efficiency, while Ion Exchange Chromatography separates molecules based on their charge. Master these techniques with our MCQs on Chromatography to enhance your exam preparation and analytical skills.
Fundamentals of Chromatography
Chromatography is primarily used for: (a) Chemical synthesis (b) Separation and analysis of mixtures (c) Conductivity measurement (d) Electrochemical reactions Answer: (b) Separation and analysis of mixtures
Which of the following is an application of chromatography? (a) Identification of compounds (b) Purification of substances (c) Determination of drug contents (d) All of the above Answer: (d) All of the above
What is the mobile phase in HPLC? (a) Gas (b) Liquid (c) Solid (d) Plasma Answer: (b) Liquid
Which detector is most commonly used in HPLC? (a) UV-Vis detector (b) Mass spectrometer (c) Refractive index detector (d) All of the above Answer: (d) All of the above
Which type of column is commonly used in HPLC? (a) Packed column (b) Open tubular column (c) Gravity column (d) None of the above Answer: (a) Packed column
In HPLC, which of the following is NOT a mode of separation? (a) Normal-phase chromatography (b) Reverse-phase chromatography (c) Paper chromatography (d) Size-exclusion chromatography Answer: (c) Paper chromatography
Ion Exchange Chromatography (IEC)
Ion exchange chromatography is mainly used to separate: (a) Neutral molecules (b) Ions based on charge (c) Proteins based on size (d) Volatile compounds Answer: (b) Ions based on charge
What is the stationary phase in ion exchange chromatography? (a) Silica gel (b) Charged resin beads (c) Gas molecules (d) Liquid solvent Answer: (b) Charged resin beads
Which type of ion exchange chromatography is used to separate anions? (a) Cation exchange chromatography (b) Anion exchange chromatography (c) Both a and b (d) None of the above Answer: (b) Anion exchange chromatography
Which factor affects ion exchange chromatography? (a) pH of the buffer solution (b) Ionic strength of the eluent (c) Temperature of the column (d) Both (a) and (b) Answer: (d) Both (a) and (b)
Which is a common application of ion exchange chromatography? (a) Water softening (b) Protein purification (c) Separation of nucleotides (d) All of the above Answer: (d) All of the above